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. 2021 Dec 9;220(2):iyab212. doi: 10.1093/genetics/iyab212

Table 1.

DNA sequences of ade6 alleles

ade6 allele Relevant sequencea
121                                                                     1461         
ade6+ (wt) AAACAAATTGA.TGGAGGACGTGAGCACAT… AGATGCCTCG
ade6-M26 AAACAAATTGA.TGGAtGACGTGAGCACAT …AGATGCCTCG
ade6-CCAAT b AAACAAATTGAtTGGAGGACGTGAGCACAT …AGATGCCTCG
ade6-Oligo-C t AACAAATTGA . accccGcac TGAGCACAT …AGATGCCTCG
ade6-M375 AAACAAATTGA.T t GAGGACGTGAGCACAT …AGATGCCTCG
ade6-M210 AAACAAATTGA.TGGAGGACGTGAGCACAT AGATGC t TCG
a

The ade6 ORF is 1,659 bp in length and coordinates are numbered relative to the first nucleotide of the start codon (+1). The hotspot (M26, CCAAT, Oligo-C), basal recombination control (M375), and test-cross (M210) alleles differ from wild-type (wt) only by the indicated bp substitutions (lower case). The bp substitutions of the hotspot alleles create DNA binding sites (grey shading) for DNA sequence-specific, hotspot-activating binding proteins (Atf1-Pcr1 heterodimer, Php2-Php3-Php5 complex, and Rst2, respectively). Each allele also renders cells auxotrophic for adenine, allowing one to score for adenine prototrophic recombinants (ade6+) from heteroallelic crosses that contain a 5’ allele (M375, M26, CCAAT, or Oligo-C) and the 3’ allele (M210).

b

The consensus motif 5’-CCAATCA-3’ is on the complementary strand.