Table 1.
DNA sequences of ade6 alleles
| ade6 allele | Relevant sequencea |
|---|---|
| 121 1461 | |
| ade6+ (wt) | AAACAAATTGA.TGGAGGACGTGAGCACAT… AGATGCCTCG |
| ade6-M26 | AAACAAATTGA.TGGAtGACGTGAGCACAT …AGATGCCTCG |
| ade6-CCAAT b | AAACAAATTGAtTGGAGGACGTGAGCACAT …AGATGCCTCG |
| ade6-Oligo-C | t AACAAATTGA . accccGcac TGAGCACAT …AGATGCCTCG |
| ade6-M375 | AAACAAATTGA.T t GAGGACGTGAGCACAT …AGATGCCTCG |
| ade6-M210 | AAACAAATTGA.TGGAGGACGTGAGCACAT … AGATGC t TCG |
The ade6 ORF is 1,659 bp in length and coordinates are numbered relative to the first nucleotide of the start codon (+1). The hotspot (M26, CCAAT, Oligo-C), basal recombination control (M375), and test-cross (M210) alleles differ from wild-type (wt) only by the indicated bp substitutions (lower case). The bp substitutions of the hotspot alleles create DNA binding sites (grey shading) for DNA sequence-specific, hotspot-activating binding proteins (Atf1-Pcr1 heterodimer, Php2-Php3-Php5 complex, and Rst2, respectively). Each allele also renders cells auxotrophic for adenine, allowing one to score for adenine prototrophic recombinants (ade6+) from heteroallelic crosses that contain a 5’ allele (M375, M26, CCAAT, or Oligo-C) and the 3’ allele (M210).
The consensus motif 5’-CCAATCA-3’ is on the complementary strand.