Figure 6.
SAX-7 and KSR-1 do not affect neurotransmitter release in ventral cord cholinergic motor neurons. (A) Whole-cell voltage-clamped body recordings from medial ventral body wall muscles (held at −60 mV in 1 mM Ca2+) of dissected worms. The graphs show the average evoked amplitude, mini frequency, and mini-amplitude at the NMJ of each strain. The number of animals analyzed per genotype is shown within each bar. Data shown as mean ± SEM. (B) Representative evoked traces (top panel) and miniature traces (bottom panel) for genotypes labeled on the top. Here, we used the sax-7(eq1) and ksr-1(ok786) alleles. P-values are calculated using one-way ANOVA with Tukey’s multiple comparison test comparing the different strains to wild-type; *P < 0.05, **P < 0.01, ***P < 0.0001.