Figure 6.

(A, B) Expression of the genes that are targets of ER-AP1-mediated transcription and AP1-independent ERE-mediated transcription in metastatic HER2-negative ER+ breast cancer patients is positively correlated with ESR2. (A) expression of ESR2 is positively correlated with CXCL12 and IGFBP4 expressions and negatively correlated with CCND1, EBAG9, and ESR1 expressions. (B) ESR1 expressions is positively correlated with PGR expressions and negatively correlated with CXCL12, E2F4, IGFBP4, and ESR2 expressions. Expression levels (FPKM) and Spearman correlation coefficients were computed for ESR1 and ESR2 expressions versus other gene expression levels. (C) Overall survival was not significantly correlated with the expression of ESR2 in the HER2-negative ERα+ metastatic breast cancer patient cohort, although there was a trend toward a positive correlation. ESR2 was dichotomized relative to the median expression level and tested via the log-rank test (p = 0.6). Cox proportional hazards regression on the continuous expression levels yielded similar results (HR 0.6, p = 0.7).