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. Author manuscript; available in PMC: 2023 Jan 28.
Published in final edited form as: Science. 2022 Jan 27;375(6579):411–418. doi: 10.1126/science.aaz5284

Fig. 3. Comparison of B cell subtypes using quantitative top-down proteomics.

Fig. 3.

Venn diagram analysis of (A) proteins and (B) proteoforms observed in pre-B-I, pre-B-II, and pre-B-III cells; naïve B cells; and memory B cells. (C) Heatmap and hierarchical clustering of quantified proteoforms from B cells subtypes. Numbers 1 to 3 represent the three major clusters of proteoforms found. (D) Volcano plot of up- and down-regulated proteoforms from naïve B cells (NB) relative to memory B cells (MB). (E) Box-and-whisker plots of proteoforms PFR1464 (TMSB4X) and PFR1215 (S100A6) levels show their variance among the five B cell subtypes. Fragmentation maps of (F) PFR1464 and (G) PFR1215. Red box indicates N-terminal acetylation. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr. (H) Distributions of proteoforms PFR1464 and PFR1215 in blood cell types of the BPA.