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. Author manuscript; available in PMC: 2022 May 12.
Published in final edited form as: Cell Rep. 2022 Apr 19;39(3):110702. doi: 10.1016/j.celrep.2022.110702

Figure 6. Atg23 dimerization is critical for the role of Atg23 in the Cvt pathway and nonselective autophagy.

Figure 6.

(A) atg23Δ cells exogenously expressing an empty vector, Atg23, or Atg23[LIL] under the endogenous promoter were grown overnight in nutrient-rich medium. Ape1 from cell lysates was inspected by western blot using anti-Ape1 antiserum.

(B) Quantification of (A).

(C) Empty vector, Atg23, or Atg23[LIL] driven by the endogenous promoter was expressed in atg23Δ cells also expressing GFP-Atg8 under the control of the CUP1 promoter. Cells were harvested in log phase after 0, 1, or 1.5 h of exposure to nitrogen-deficient medium. Processing of GFP-Atg8 to free GFP was assessed by western blot.

(D) Quantification of (C).

(E) Pho8Δ60 activity was assessed for atg23Δ Pho8Δ60 cells expressing empty vector, Atg23, or Atg23[LIL] under the control of the endogenous promoter. Quantification shows values as a percentage of wild-type activity after 4 h of nitrogen starvation.

(B, D, and E) Error bars represent SEM; n = 3; ***p < 0.001 by Tukey HSD test.