Fig. 2.

LINC00662 regulates BC cell progression by competitively binding to miR-144-3p. A Cytoplasmic and nuclear fraction RNA analysis was applied to detect LINC00662 location. B Putative miRNAs which share binding sites with LINC00662 were screened from starBase V2.0. C QRT-PCR analysis exhibited relative miRNAs expression after knockdown of LINC00662. D RIP assay examined relative RNA expression. E Binding sites between LINC00662 wild type (LINC00662 WT) and miR-144-3p and the base sequence of LINC00662 mutant type (LINC00662 Mut) were demonstrated. Luciferase reporter assay verified the putative binding sites between LINC00662 and miR-144-3p. F CCK-8 assay was carried out to evaluate how cell proliferative ability changed after knockdown of LINC00662 and knockdown of LINC00662 and miR-144-3p both. G–I Transwell, sphere formation, and western blot assays were conducted to examine that inhibited cell migration, invasion, stemness ability and stemness-related protein level by knockdown of LINC00662 were rescued by knockdown of miR-144-3p. ^*p < 0.05; ^**p < 0.01