Fig. 3.

LINC00662 targets miR-144-3p/SOX2 axis to modulate BC cell progression. A QRT-PCR analysis was employed to investigate the expression of stemness-related genes in MCF-10A, MDA-MB-231, MCF-7, MDA-MB-468 and MDA-MB-453 cells. B Binding sites between SOX2 wild type (SOX2 WT) and miR-144-3p and the base sequence of SOX2 mutant type (SOX2 Mut) were demonstrated. C CCK-8 assay evaluated cell proliferative ability after the down-regulation of LINC00662 or knockdown of LINC00662 and overexpression of SOX2. D–F Transwell, sphere formation, and western blot assays were conducted to examine cell migration, invasion, stemness ability and stemness-related protein level after the down-regulation of LINC00662 or knockdown of LINC00662 and overexpression of SOX2. G RIP assay evaluated the enrichments of LINC00662, miR-144-3p, SOX2 in RISC in BC cells. H Luciferase reporter assay was carried out to verify the ceRNA network among LINC00662, miR-144-3p and SOX2. ^** p < 0.01; ^*** p < 0.001