Figure 6.

SPARTA depletes plasmid-harboring cells from bacterial cultures

(A) Graphical schematic representation of the mixed-culture competition experiment (B).

(B) Cultures of MapSPARTA-expressing E. coli cells that contain pUC-empty or those that were plasmid-free were mixed in a 24:1 ratio, cultivated in LB, and the percentage of plasmid-containing cells was determined daily. Cultures of E. coli cells with an empty BAC or a BAC expressing MapSPARTA^TIR-E77A were used as control. Graph shows the average of six biological replicates.

See also Figure S6.