Figure S5.

Plasmid DNA triggers SPARTA-mediated NAD(P) depletion and cell death, related to Figure 5

(A) Plasmid DNA triggers SPARTA-mediated NAD(P) depletion and cell death. NAD and NADP levels and viability were determined in E. coli cultures expressing MapSPARTA or MapSPARTA^TIR-E77A in the presence or absence of the high copy pUC-empty plasmid. Panels showing NAD levels at t = 4 and cell viability at t = 20 are identical to those in Figure 5A.

(B) Native RNA polymerase-mediated transcription of plasmid-encoded genes enhances SPARTA-mediated cell death. Cell viability was determined in E. coli cultures expressing MapSPARTA or MapSPARTA^TIR-E77A either in the absence of plasmid DNA or in the presence of a pUC-derivate from which mRFP is transcribed but not translated (pUC-mRFP^XylS-Pm) or encoded but not transcribed (pUC-mRFP^XylS-ΔPm). The Pm promoter induces transcription by native RNA polymerases. Graphs show the average of three biological replicates. Error bars indicate standard deviations. n.s = not significant; ^∗∗p < 0.01; ^∗∗∗p < 0.001.