Fig. 4. Influence of miR-152 expression on the suppressive role of OIP5-AS1 knockdown in ox-LDL-treated hVSMCs. hVSMCs were transfected with si-OIP5-AS1 or si-con, and co-transfected with miR-152 inhibitor (anti-miR-152) or miR-control inhibitor (anti-miR-con), and then treated with 100 μg mL−1 of ox-LDL or not. (A) RT-qPCR detected miR-152 expression level at 24 h. (B) CCK-8 measured cell proliferative capacity at 0 h, 24 h, 48 h and 72 h. (C) Western blotting examined PCNA expression at 24 h. (D) Wound healing assay determined the ability of cell migration at 48 h. (E) Western blotting examined expression of MMP-2 and MMP-9 at 24 h. Data represent mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 according to one-way ANOVA.
