Fig. 6. Impact of PAPPA expression on biological role of miR-152 overexpression in hVSMCs. hVSMCs were transfected with miR-152 alone or together with plasmid pcDNA-PAPPA (PAPPA) or pcDNA, and then treated with 100 μg mL⁻¹ of ox-LDL. (A) Western blotting detected PAPPA expression level at 24 h. (B) CCK-8 measured cell proliferation at 0 h, 24 h, 48 h and 72 h. (C) Western blotting examined PCNA expression at 24 h. (D) Wound healing assay determined the migration rate at 48 h. (E) Western blotting examined expression of MMP-2 and MMP-9 at 24 h. Data represent mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 according to one-way ANOVA.