| Antibodies |
|
| Anti-MISP |
Thermo Fisher Scientific |
Cat# PA5-61995; RRID:AB_2638859 |
| Anti-villin |
Santa Cruz Biotechnology |
Cat# sc-66022; RRID:AB_2216247 |
| Anti-ezrin |
Cell Signaling Technology |
Cat# 3145; RRID:AB_2100309 |
| Anti-GAPDH |
Cell Signaling Technology |
Cat# 2118; RRID:AB_561053 |
| Anti-β-actin |
Sigma-Aldrich |
Cat# A5316; RRID:AB_476743 |
| F(ab’)2-Goat anti-rabbit Alexa Fluor 488 |
Molecular Probes |
Cat# A-11070; RRID:AB_142134 |
| F(ab’)2-Goat anti-mouse Alexa Fluor 568 |
Molecular Probes |
Cat# A-11019; RRID:AB_143162 |
| IRDye 800CW Donkey anti-rabbit IgG antibody |
LI-COR Biosciences |
Cat# 926-32213; RRID:AB_621848 |
| IRDye 800CW Donkey anti-mouse IgG antibody |
LI-COR Biosciences |
Cat# 926-32212; RRID:AB_621847 |
|
| Bacterial and virus strains |
|
| DH5-Alpha competent Escherichia coli cells |
Molecular Cell Biology Resource Core, Vanderbilt University Medical Center |
Cat# DH5-Alpha |
|
| Chemicals, peptides, and recombinant proteins |
|
| Blasticidin |
Gold Biotechnology |
Cat# B-800 |
| G418 Disulfate |
Gold Biotechnology |
Cat# G-418 |
| Phleomycin |
InvivoGen |
Cat# Ant-ph-1 |
| Doxycycline |
RPI |
Cat# D43020
|
| Puromycin |
Gold Biotechnology |
Cat# P-600-100 |
| NSC668394 |
Sigma-Aldrich |
Cat# 341216 |
| DMSO |
Sigma-Aldrich |
Cat# D8418 |
| TET-free FBS |
Atlanta Biological |
Cat# S10350
|
| FuGENE 6 |
Promega |
Cat# E2691 |
| Polybrene Infection Reagent |
Sigma-Aldrich |
Cat# H9268 |
| Lipofectamine 2000 |
Thermo Fisher Scientific |
Cat# 11668019 |
| Lenti-X Concentrator |
Clontech |
Cat# 631231 |
| Paraformaldehyde, 16% |
Electron Microscopy Sciences |
Cat# 15710 |
| ProLong Gold Antifade Mountant |
Invitrogen |
Cat# P36930
|
| cOmplete, Mini Protease Inhibitor Cocktail |
Roche |
Cat# 04693124001 |
| cOmplete ULTRA Tablets, Protease Inhibitor Cocktail |
Roche |
Cat# 5892953001 |
| Ni-NTA magnetic beads |
NEB |
Cat# S1423 |
| TEV protease |
NEB |
Cat# P8112 |
| Rabbit skeletal G-actin |
Cytoskeleton Inc |
Cat# AKL99 |
| Poly-L-lysine solution, 0.1% |
Sigma-Aldrich |
Cat# P8920 |
| Carbon-coated copper grids |
Electron Microscopy Sciences |
Cat# CF300-Cu |
| Alexa Fluor 568 Phalloidin |
Invitrogen |
Cat# A12380 |
| Alexa Fluor 647 Phalloidin |
Invitrogen |
Cat# A22287 |
| Wheat Germ Agglutinin 405M (WGA) |
Biotium |
Cat# 29028-1 |
| DRAQ5 Fluorescent Probe Solution |
Thermo Fisher Scientific |
Cat# 62251 |
|
| Experimental models: Cell lines |
|
| LS174T-W4 |
Gift from Dr. Hans Clevers (Utrecht University, Netherlands) |
Baas et al. (2004); RRID CVCL J074 |
| CACO-2BBE
|
Gift from Dr. Mark Mooseker (Yale University) |
N/A |
| LLC-PK1-CI4 |
Gift from Dr. Carolyn Slayman (Yale University) |
N/A |
| HEK293T |
ATCC |
CRL-11268 |
| HeLa |
ATCC |
CCL-2 |
| Sf9 |
Thermo Fisher Scientific |
Cat# 11496015 |
|
| Experimental models: Organisms/strains |
|
| Mus musculus (C57BL/6J) |
Jackson Laboratory |
000664 |
|
| Recombinant DNA |
|
| pCMV-SPORT-MISP |
Harvard PlasmID Database |
HsCD00326629 |
| pEGFP-C1-MISP |
This paper |
N/A |
| pmCherry-C1-MISP |
This paper |
N/A |
| pHALO-C1-MISP |
This paper |
N/A |
| pEGFP-C1-fimbrin |
Tyska Laboratory |
N/A |
| pEGFP-C1-villin |
Tyska Laboratory |
N/A |
| pEGFP-C1-espin |
Gift from Dr. James Bartles (Northwestern University) |
N/A |
| pHALO-C1-β-actin |
Tyska Laboratory |
N/A |
| pEGFP-N1-ezrin |
Addgene |
Cat# 20680 |
| PLKO.1 (scramble) |
Dr. David Sabatini / Addgene |
Cat# 1864 |
| PLKO.1 (MISP-KD) |
Sigma-Aldrich |
TRCN0000422523 |
| PLKO.1 (MISP-KD-2) |
Sigma-Aldrich |
TRCN0000116527 |
| psPAX2 packaging plasmid |
Dr. Didier Trono / Addgene |
Cat# 12260 |
| pDMD2.G |
Dr. Didier Trono / Addgene |
Cat# 12259 |
| pFastBac-6xHis-MBP |
Addgene |
Cat# 30116 |
| pFastBac-6xHis-MBP-MISP |
This paper |
N/A |
| pFastBac-6xHis-MBP-EGFP-MISP |
This paper |
N/A |
| pLVX-EGFP |
This paper |
N/A |
| pLVX-EGFP-MISP |
This paper |
N/A |
|
| Software and algorithms |
|
| FIJI |
https://fiji.sc
|
N/A |
| NIS AR Elements Analysis |
Nikon (https://bit.ly/3fFd8rz) |
N/A |
| Prism 8.1.2 |
GraphPad (https://graphpad.com) |
N/A |
|
| Other |
|
| 35 mm glass bottom dishes |
Invitro Scientific |
Cat# D35-20-1.5-N |
