Figure 4.

Active androgens are produced in adrenal glands and progesterone metabolism is activated in the Enza-treated tumors

The high adrenal-to-serum steroid ratios indicate the production of active androgens in the mouse adrenal gland (A), and the low tumor-to-serum ratios in mice treated with Enza (ORX + Enza I and ORX + Enza II) indicate the activation of progesterone (P₄) metabolism in tumors treated by Enza, while in other treatment groups, the ratio was close to 1 (B). Kruskal-Wallis with Dunn’s post hoc test was used for statistical analyses. Data are expressed as the median and range with Tukey box and whisker plots (A–C). n = 17 in ORX I and ORX II, n = 14 in ORX + Enza I and II, n = 15 in ORX + ADX I and n = 11 in ORX + ADX II (A–C). ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.