The kinetic of LD and lysosome intracellular distributions are correlated and dependent of oleate concentration
(A) Huh7 cells were grown overnight. Then culture medium was supplemented without or with 50, 100, 200, and 400 μM sodium oleate complex for different time (12, 24, 48, and 72 h), then stained for LDs (green) and LAMP1(red). Scale bar, 20μm.
(B) LDs intensity were analyzed in 25 cells from three experiments performed as described in (A).
(C) The percentage of LD intensity in perinuclear and peripheric are present in 25 cells from three experiments performed as described in (A).
(D) LAMP1 intensity of 25 cells from 3 independent experiments performed as described in (A).
(E) The percentage of LAMP1 intensity in perinuclear and peripheric are present in 25 cells from three experiments performed as described in (A). Data information: Linegraphs present Mean ± SEM. Student’s t-test was used. ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.0001.