Figure 5.

Septin 9 is associated with large LDs in the perinuclear area

(A) Huh7 cells were grown overnight. Then culture medium was supplemented without or with 100 and 400 μM sodium oleate complex for different time (24 h, 72 h), then stained for LDs(red) and septin 9 (green). Scale bar, 20μm.

(B) Dot rectangles in (A) are presented in higher magnification. Scale bar, 5μm. Bar graphs show total intensity of LD and Mean intensity of Rab7 on LDs. Data information: Bar graphs present Mean ± SEM. Student’s t test was used. ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.0001.