REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Phospho-eIF2a_Ser51	Cell Signaling Technology	Cat# 3597; RRID:AB_390740
EIF2ΑK1/HRI	Proteintech	Cat# 20499-1-AP; RRID:AB_10697665
EIF2ΑK1/HRI	Abcam	Cat# ab28530; RRID:AB_869595
Anti-Bag3	Proteintech	Cat# 10599-1-AP; RRID:AB_2062602
β-actin	Novus	Cat# NB600-501; RRID:AB_10077656
a-K48 Ubiquitin linkage antibody	BostonBiochem	Cat#A101-050; RRID:AB_2894847
Chemicals, peptides, and recombinant proteins
JG98	AdooQ BIOSCIENCE	Catalog No.: A18734
MG132	Biomol	LKT-M2400.5
DAPI (4′,6-Diamidine-2′-phenylindole dihydrochloride)	Sigma	Cat# 10236276001
Formaldehyde	Sigma	Cat#FX0415
Blasticidin S HCl	Thermo Fisher	Cat# A1113903
Recombinant Human His6-Ubiquilin-1 Tandem UBA	BostonBiochem	UBE110
Qiagen RNeasy Plus Mini kit	Qiagen;GmbH	Cat. #74134
qScript cDNA Synthesis Kit	Quanta Bio	Cat. #95047-100
PerfeCTa SYBR Green FastMix ROX	Quanta Bio	Cat. #95073-012
DMEM/F-12 1:1	BI-Biologicals	Cat# 01-170-1A
horse serum	BI-Biologicals	Cat. #04-0041A
epidermal growth factor	Peprotech	Cat. #AF-100-15
Hydrocortisone	Sigma	Cat. #H-0888
human insulin	Sigma	Cat. #I9278
cholera toxin	Sigma	Cat. #C-8052
Fetal Bovine Serum	BI-Biologicals	Cat. #04-007-1A
L-glutamine	BI-Biologicals	Cat. #03-020-1B
L-alanyl-L-glutamine	BI-Biologicals	Cat. #03-022-1B
Penstrep	BI-Biologicals	Cat. #03-031-1B
HisPur Ni-NTA Resin	ThermoFisher Scientific	Cat. #88221
RNeasy Mini kit	Qiagen	Cat. #74104
Deposited data
Transcriptome analysis	GEO	GSE171440
Experimental models: cell lines
MCF10A (human breast epithelial)	ATCC	Cat# CRL-10317; RRID:CVCL_0598
HEK293T	ATCC	Cat# CRL-3216, RRID:CVCL_0063
Oligonucleotides
Supplement table	See Tables S3 and S4	N/A
Software and algorithms
Hermes Wiscon Imaging System with Athena Winsoft software package (V1.0.10)	IDEA Bio-Medical Ltd	RRID:SCR_021786
Fiji-ImageJ	ImageJ	RRID:SCR_002285
limma package	R-package	LIMMA, RRID:SCR_010943
Gene Set Enrichment Analysis	Broad Institute	RRID:SCR_003199
GraphPad Prism_v9	GraphPad Software Inc	GraphPad Prism, RRID:SCR_002798
trim_galore_v0.5.0	Babraham Bioinformatics	Trim Galore, RRID:SCR_011847
FastQC_v0.11.7	Babraham Bioinformatics	FastQC, RRID:SCR_014583
Hisat2_v2.1.0	daehwankimlab	HISAT2, RRID:SCR_015530
GRch38.97	UCSC	NA
samtools_v1.9	N/A	SAMTOOLS, RRID:SCR_002105
featureCounts_v1.6.3	N/A	featureCounts, RRID:SCR_012919
Bioconductor packages	Bioconductor	RRID:SCR_006442