Figure 3.

Changes in new protein synthesis and transport to the optic nerve at 5 d after injury. a, Comparison of proteins in optic nerve samples shows 118 of 302 (39%) AHA-labeled proteins detected in both experimental replicates. b, Scatter plot of peptide ratios of crush/control of two optic nerve (ON) replicates shows tight correlation between samples (Pearson's r = 0.914). Histograms of crush/control ratios of replicate 1 (above) and replicate 2 (right) shows distribution of synthesized and transported proteome. Full data are available in Extended Data Figure 3-1. c, Z-score comparison of crush compared with control, highlighting the largest changes in transport (average Z-score > 1.5) of newly synthesized, AHA-labeled proteins into the optic nerve 5 d after injury. Gene Ontology analysis of compartmentalization of optic nerve proteins is available in Extended Data Figure 3-2.