Figure 5.

p57 overexpression in stomach epithelium prevents activation of chief cells after injury

(A) Scheme of the in vivo experiment.

(B–D) (B) p57, (C) Ki67, and (D) Gif staining in control epithelium without injury (left), control at 3 dpi with HDT (middle), and p57-OE epithelium at 3 dpi with HDT (right). Rectangles indicate insets showing base region. Scale bars, 100 μm (upper figures) and 20 μm (lower figures).

(E) Triple staining with markers for chief cells (Gif, magenta), neck cells (GSII, green), and proliferating cells (Ki67, white) in the conditions as outlined above. Scale bars, 100 μm.

(F) Insets of figure (E). Scale bars, 20 μm.

(G) Quantification of injury-responsive cells (Gif⁺/GS-II⁺, Gif⁺/Ki67⁺, GS-II⁺/Ki67⁺, and Gif⁺/GS-II⁺/Ki67⁺). Control UT, Anxa10-CreERT^2/+ untreated; control d3, Anxa10-CreER^T2/T2 HDT d3; p57 OE d3, Anxa10-CreER^T2/T2; R26loxpTA-p57^k/k HDT d3. In total, 16–20 glands from 2–3 mice per condition were analyzed. ^∗∗∗∗p < 0.0001 calculated by one-way ANOVA for total injury-responsive cell number.

See also Figure S5.