Skip to main content
. Author manuscript; available in PMC: 2022 May 12.
Published in final edited form as: Oncogene. 2019 Feb 12;38(23):4540–4559. doi: 10.1038/s41388-019-0736-3

Figure 5. Prostate cancer-activated MLO-Y4 cells promote C4-2B prostate cancer cell proliferation, migration and invasion through EGR1 in the prostate cancer cells.

Figure 5.

(A). Confirmation of siRNA-mediated EGR1 protein expression knockdown in C4-2B cells. Total cell protein lysate was subjected to immunoblot for EGR1. (B). C4-2B cells (1.5×104 per well) were treated with siControl or siEGR followed by C4-2B (left) or PC3 (right) CM-treated MLO-Y4 cell CM (or plain media as control). After 48 hours, cell numbers were counted using a hemocytometer. (C and D). Migration (upper figures) and invasion (lower figures) were assessed using a transwell assay. Both siControl-treated and siEGR-treated C4-2B cells (2×105 per cells) were treated with C4-2B (C) or PC3 (D) CM-treated MLO-Y4 cell CM, as indicated, for 24 hours. The membrane was stained using differential Quick staining kit and photographed under light microscopy (20x). The numbers of migrating (no Matrigel on membrane) and invading (Matrigel present on membrane) cells were counted in five random fields for each insert. Data are shown as the mean±SD of 3 independent experiments. * P < 0.05; ** P < 0.01.