Figure 6.

Mllt11 associated and colocalized with acetylated α-tubulin in growing neurites. A, Co-IP of acetylated α-tubulin (Actub) with Myc-tagged Mllt11 in whole cell lysates of HEK293 cells compared with a myc programmed control. B, Co-IP probed with Mllt11 antibody showing IP signal restricted to lanes containing Mllt11. C, D, Primary cortical neurons cultured for 24 h (C) or 7 d (D) postplating. C, After 24 h, Mllt11 colocalizes with Tubb3 in the growth cone and at swellings along the distal axon as indicated by arrowheads (c'), and in the soma (C). After 7 d, Mllt11 was primarily found along proximal portions of the axon where it colocalizes with acetylated α-tubulin (D). E, Coronal sections of the corpus callosum (cc) at E18.5 labeled with acetylated α-tubulin and Tubb3. Brackets show decreased thickness in cKO compared with control. Student's t test with Welch's correction, (A, B) N = 4 (controls and cKOs). Data presented as mean ± SD n.s., not significant (i.e., p > 0.05). Scale bar: 20 μm (C, D) and 50 μm (E).