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. 2022 Apr 3;26(10):2895–2907. doi: 10.1111/jcmm.17304

FIGURE 6.

FIGURE 6

LncRNA FAF exerted antipyroptotic effects by targeting miR‐185‐5p/PAK2. (A and B) The differential expression of miR‐185‐5p and PAK2 between hypoxic and normoxic cardiomyocytes. (C) The protein expression of PAK2 in hypoxic NRCM transfected with Adv‐FAF and/or miR‐185‐5p mimics was determined by Western blots. n=3, **p < 0.01, vs the control group, # vs the Adv‐FAF+mimic‐miR‐185‐5p+hypoxia group. (D‐E) Transfection of miR‐185‐5p inhibitor contributed to downregulating the pyroptosis‐related protein expression, whereas miR‐185‐5p mimic transfection upregulated pyroptosis‐related protein expression. n=3, *p < 0.05, **p < 0.01, vs the inhibitor NC or mimic NC group, #p < 0.05, ##p < 0.01 vs the inhibitor NC+hypoxia group or mimic NC+hypoxia group. (F‐G) Transfection of miR‐185‐5p mimics or FRAX597 abolished the antipyroptotic effects of lncRNA FAF overexpression in hypoxic NRCM. n = 3, *P*p < 0.05, **P**p < 0.01, vs the control group, #p < 0.05, ##p < 0.01 vs the Adv‐FAF +mimic‐miR‐185‐5p + hypoxia group or Adv‐FAF +FRAX597 + hypoxia group. (H) The infarct size of rats after regulating miR‐185‐5p/PAK2 axis was measured by TTC staining. n = 5 rats per group, ***p < 0.001, vs the MI +Adv‐FAF +mimic‐NC group; ##p < 0.01 vs the MI +Adv‐FAF +FRAX597 group