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. 2022 Apr 26:eabn7737. doi: 10.1126/scitranslmed.abn7737

Fig. 5. Engineered ACE2 neutralizes authentic Omicron and confers protection in hamsters and hACE2 transgenic mice.

Fig. 5.

(A) Sensitivity of engineered ACE2 to the Wuhan and Omicron strains was compared by using each infectious virus in Vero E6/TMPRSS2 cells. RNA copy number was analyzed by qRT-PCR against nucleocapsid. n = 3 technical replicates. (B) Experimental scheme for the hamster model of SARS-CoV-2. (C) Quantification of viral RNA in the lungs of treated or untreated hamsters at day 5 post infection with the SARS-CoV-2 Omicron strain was performed by qRT-PCR against nucleocapsid. (n=4 per group). (D) Gene expression of inflammatory cytokines and chemokines was quantified by qRT-PCR in the hamsters. The expression of the gene encoding β-actin was used for normalization. (n=4). (E) A survival curve is shown for CAG-hACE2 Tg mice infected with the Omicron strain with or without 3N39v4 ACE2 decoy treatment (control: 2 male, 2 female, treated: 3 male and 3 female). P-values were determined by Mann-Whitney U test (C), one-way ANOVA and Tukey's multiple comparison test (D) and log-rank test (E).