Fig 3. Effects of substitutions in the envelope protein.

a) Substitutions with non-zero effect sizes with 95% interquartile range across the 100-fold Monte Carlo cross-validations as whiskers, median as points. Points are colored red if they match positions of known epitopes for monoclonal antibodies compiled in the DENVab database [24]. Gray vertical lines indicate positions with known human-derived monoclonal antibody (hmAb) epitopes, long if within site diversity exists in our dataset and short if not. b) Footprints of potently neutralizing hmAbs, colored red if the positions showed non-zero effects. c) Top and side views of the envelope protein structure with known epitopes colored red if estimated as non-zero effect, and gray if estimated as zero effect. Non-zero effect positions not matching reported hmAb epitopes are in black.