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. 2022 Apr 21;82(8):1557–1572.e7. doi: 10.1016/j.molcel.2022.01.019

Figure 2.

Figure 2

The ASCC accumulates on UVB- and 4NQO-stalled ribosomes

(A) Harringtonine run-off assays to measure the rate of polysome loss in HeLa cells treated with 4NQO or UVB. Error bars represent the SD. ∗∗p < 0.01, ∗∗∗p < 0.001.

(B) Micrococcal nuclease assay to measure ribosome collisions in HeLa cells treated with 2-μM emetine (low), 0.2-μM anisomycin (low), 300-μM emetine (high), 75-μM anisomycin (high), 4NQO, or UVB for 15 min.

(C) Distribution of the ASCC proteins after SDGC of lysates prepared from control or 4NQO-treated cells.

(D and E) ASCC3 distribution after SDGC of lysates prepared from cells exposed to 4NQO. Lysates were treated with EDTA (+EDTA) in (D) or MNase (+MNase) in (E).

See also Figure S2.