Skip to main content
. 2022 Mar 8;11(3):1114–1128. doi: 10.1021/acssynbio.1c00342

Figure 1.

Figure 1

Drying systems to preserve active cell-free extracts. (A) Low-cost drying: low-vacuum, room-temperature drying apparatus using silica beads. (B) High-cost lyophilization: commercial, computer-controlled freeze dryer for lyophilization (FreeZone Triad Benchtop Freeze Dry System -Labconco). (C) Percentage of recovery after 1 day and 2 weeks of storage at room temperature in a sample. Cell-free reactions based on PEP (top panel) or MDX (bottom panel) as energy sources. Plasmid psfGFP was used as a DNA template. Percentage of recovery was calculated relative to the RFU value obtained from fresh extracts with the respective energy sources (PEP or MX). Cell-free reactions were incubated at 29 °C for 15 h. Error bars represent standard error over 12 technical measurements.