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. 2022 Mar 8;11(3):1114–1128. doi: 10.1021/acssynbio.1c00342

Figure 3.

Figure 3

Enhancer effect of lactose on gene expression using linear DNA templates in cell-free reactions. CFPS based on (A) PEP or (B) MDX. Details of additives in each cell-free mixture are shown in Table S5. GamS and Chi6 were added at a final concentration of 2 μM. Except for NTC (no template control), all reactions contained 5 nM DNA (plasmid or linear). Linear DNA templates were amplified with extended 100 bp flanks to protect the template (highlighted with a gray dashed box). Unprotected linear DNA template was amplified with extended 3 bp flanks (denoted as “short flanks”). Black strips are representative images of the fluorescence signal on the plate captured using an imaging system (BioRad GelDoc-Go). Cell-free reactions were incubated at 29 °C for 10 h. All error bars represent standard error over three biological replicates based on three technical measurements.