Fig. 1 |. cbDVg screening methods and sensitivity.
a, Scheme of the PCR strategy for cbDVG detection. Le: leader sequence on the 3′-untranslated region (UTR) of the viral genome; Tr: trailer sequence on the 5′ UTR of the viral genome; P1: specific primer used in both reverse transcription and PCR; P2: specific primer used only during PCR. b, Representative PCR results for seven paediatric samples. Five cbDVG-positive and two cbDVG-negative samples are shown. c, Graph of cbDVG reads normalized to 108 total reads obtained from our RNA-seq/VODKA pipeline.