Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information | |
|---|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK293 | ATCC | CRL-1573.3 | Mycoplasma tested negative | |
| Cell line (H. sapiens) | HEK293T | ATCC | ACS-4500 | Mycoplasma tested negative | |
| Cell line (H. sapiens) | HeLa | ATCC | CRM-CCL-2 | Mycoplasma tested negative | |
| Antibody | Anti-dopamine receptor D1(rabbit polyclonal) | Abcam | ab216644 | IF (1:100) | |
| Antibody | Anti-GM130 (mouse monoclonal) |
BD Biosciences | 610822 | IF (1:1000) | |
| Antibody | Anti-SLC22A2 (rabbit polyclonal) | Abcam | ab170871 | WB (1:1000) | |
| Antibody | Anti-SLC22A2 (rabbit polyclonal) | ABclonal | A14061 | IF (1:100) | |
| Antibody | Anti-GAPDH (mouse monoclonal) |
Proteintech | 60004-1 | WB (1:10,000) | |
| Antibody | Anti-HRP-conjugated IgG (rabbit polyclonal) | GE Healthcare | P132460 | WB (1:10000) | |
| Antibody | Anti-mouse IgG (donkey polyclonal) |
Thermo Fisher | A32766 | IF (1:10,000) | |
| Antibody | Anti-rabbit IgG (donkey polyclonal) |
Thermo Fisher | A32794 | IF (1:10,000) | |
| Antibody | Anti-dopamine receptor D1 (rabbit polyclonal) | Proteintech | 17934-1-AP | IF (1:100) | |
| Peptide, recombinant protein | D1DR blocking peptide | Proteintech | Ag12366 | IF (1:25) | |
| Chemical compound, drug | Snap-Cell 647 | NEB | S9102S | ||
| Chemical compound, drug | Dopamine hydrochloride | Sigma | 200-527-8 | ||
| Chemical compound, drug | SKF81297 hydrobromide | Tocris | 1447 | ||
| Chemical compound, drug | NPEC-caged-dopamine | Tocris | 3992 | ||
| Chemical compound, drug | A/C heterodimerizer | Takara | 635056 | ||
| Chemical compound, drug | Dyngo | Abcam | ab120689 | ||
| Chemical compound, drug | Forskolin | Sigma | F6886-10MG | ||
| Chemical compound, drug | Imipramine | Sigma | 113-52-0 | ||
| Chemical compound, drug | Corticosterone | Sigma | 200-019-6 | ||
| Software, algorithm | Prism | GraphPad | |||
| Software, algorithm | ImageJ | Imagej.net/contributors | |||
| Software, algorithm | MATLAB R2014b | MathWorks | DOI:10.5281/zenodo.5146169 | Bakr et al., 2021 | |
| Recombinant DNA reagent | pGloSensor-20F | Promega | E1171 | ||
| Recombinant DNA reagent | Signal sequence Snap-D1DR | This study | pCDNA3 backbone; snap vector, see Materials and methods | ||
| Recombinant DNA reagent | pVenus-FRB-Nb6B9 | This study | pVenus-C1 vector, see Materials and methods | ||
| Recombinant DNA reagent | FKBP-GalT-mApple | This study | pm-Apple-M1 vector, see Materials and methods | ||
| Recombinant DNA reagent | Lyn-2xFKBP-CFP | Addgene | 20149 | ||
| Recombinant DNA reagent | pCAG-Snap-D1DR | This study | pCAG vector, see Materials and methods | ||
| Recombinant DNA reagent | pCAG-Nb6B9-GFP pCAG-Nb37-GFP | This study | pCAG vector, see Materials and methods | ||
| Sequence-based reagent | SLC22A2 shRNAs | OriGene | TL517269 | pGFP-C-shLenti | |
| Sequence-based reagent | Scamble shRNAs | OriGene | TR30021 | pGFP-C-shLenti | |
| Biological sample (Mus musculus, male and female) | CD1 | Charles Rivers | Crl:CD1(ICR) | Isolated medium spiny neurons from neonatal mouse striatal, see Material and methods | |