Figure 3.

SH003 combined with DTX inhibits EGFR activation and EGFR downstream pathways. Cells were treated with SH003, DTX, or combination for 30 min. (a, b) Western blotting of EGFR in BT-20 and MDA-MB-231 cells. The bar graph indicates the relative protein expression and normalized to total EGFR. (c, d) Western blot analysis of EGFR downstream pathways in BT-20 and MDA-MB-231 cells. The relative expression of proteins was measured by Image J software. The data represent mean ± SD on three independent experiments. ∗P < 0.05, ∗∗P < 0.01 vs. control.