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. 2022 May 12;13:2638. doi: 10.1038/s41467-022-30303-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a The influence of MRNIP phase separation on HR-mediated DNA repair was explored with an DSB repair reporter. Data are presented as means ± SEM; n = 3 biological replicates. b, c Comet assays were conducted to explore the impact of MRNIP depletion on DNA repair. Data are presented as means ± SEM. b n = 58, 52, 62, 45, 59, 69 (from left to right column). c n = 76, 71, 98 (from left to right column). d, e γ-H2A.X was detected by immunofluorescence assay, and the γ-H2A.X content was counted. Data are presented as means ± SEM. d n = 48, 51, 96, 182, 164, 199, 103, 116 (from left to right column). e n = 50, 50, 50, 75, 63, 73, 81, 62, 77, 190, 141, 173 (from left to right column). f, g Colony formation assay showed that MRNIP-enhanced tumor radioresistance was ascribed to its LLPS capacity. Data are presented as means ± SEM; n = 3 biological replicates. For (a, c, e, g), HeLa-KO-MRNIP cells stably expressing sgRNA-resistant MRNIP-GFP, MRNIP-ΔIDR1-GFP (ΔIDR1) and empty vector (EV) were used. h IDR of FUS restored the LLPS capacity of MRNIP-ΔIDR1. HeLa-KO-MRNIP cells were transfected with plasmid for 24 h before observation. i FUSN-ΔIDR1 increased RPA1 foci after radiation in HeLa-MRNIP-KO cells. Data are presented as means ± SEM; n = 3 biological replicates. j FUSN-ΔIDR1 accelerated radiation-induced γ-H2A.X accumulation and DNA repair. Data are presented as means ± SEM. Left panel: n = 96, 79, 79, 102, 64, 63 (from left to right column). Right panel: n = 48, 45, 92, 128, 163, 176, 126, 131 (from left to right column). k The impact of FUSN-ΔIDR1 on HR was analyzed using DSB repair reporter. Data are presented as means ± SEM; n = 3 biological replicates. For (h–k), HeLa-KO-MRNIP cells stably expressing sgRNA-resistant MRNIP-ΔIDR1-GFP (ΔIDR1) and FUSN-MRNIP-ΔIDR1-GFP (FUSN-ΔIDR1) were used. All P values were calculated using two-tailed unpaired Student’s t test. ns, no significance.