Fig. 4.

Effects of CX-5461 on cell cycle progression in SK-UT-1 cells (a) Flow cytometry gating strategy for cell cycle analysis using BrdU and PI staining at 0 and 72 h following treatment with 30 nM CX-5461, or vehicle, is shown. (b) SK-UT-1 cells were treated with the GIC₅₀ CX-5461 dose (30 nM) for the times indicated, and stained with a anti BrdU specific antibody and PI for cell cycle analysis as in (a) (bars represent mean ± SD of n = 2 biological duplicates). *V-72 h: Vehicle for 72 h