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. 2022 Jan 28;40(3):506–518. doi: 10.1007/s10637-022-01218-6

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — (A, B) Population of CD11b⁺Gr-1⁺ MDSCs in the spleen, PB, BM, and LP by flow cytometry five, seven and eight weeks post-induction (W5, W7 and W8) in the CAC group and the normal control group (NC, A) and in the MyD88 inhibitor-treated group (I, B). N = 5 per group. C&D) IHC staining for Ly6G⁺ cells of colon sections (C) and IF staining for CD11b⁺ (green) Ly6G⁺ (red) cells of spleen sections (D) in the CAC group and the I group five, seven and eight weeks post-induction (W5, W7 and W8). Scar bar = 50 μM. Histogram at the bottom (C) shows the percentage of positive Ly6G⁺ cells in total cells. N = 4 per group. Data are expressed as the mean ± SD of each group. ***P < 0.001