Fig. 2.

MyD88 inhibitor treatment reduced the expression of factors associated with MDSC accumulation in mice with CAC. A) Population of G-CSF, IL-6 and TGF-β-producing CD3⁻ splenocytes by flow cytometry eight weeks post-induction. B) Relative levels of GM-CSF, IL-1β, IL-6 and TGF-β mRNA transcripts in mouse colons eight weeks post-induction examined by RT-qPCR. Data are expressed as the mean ± SD of each group. All data of RT-qPCR were normalized to that in normal control mice. **P < 0.01; ***P < 0.001. N = 5 per group. Groups: the normal control (NC), CAC and MyD88 inhibitor-treated (I) groups