Fig. 3.

Impact of MyD88 inhibitor on the expression of molecules involved in MDSC-mediated immune suppression in mice with CAC. A) Protein extracts from colon tissue were analyzed by Western blotting for iNOS, Arg-1 and IDO. α-tubulin was used as a control. Data are representative images or expressed as the mean ± SD of each group. Densitometry analysis was performed with Image J software for quantification. **P < 0.01. B) Representative IHC stains for iNOS, Arg-1 and IDO in colonic samples seven weeks post-induction. Scale bar = 100 μM. N = 4 per group. Groups: the normal control (NC), CAC and MyD88 inhibitor-treated (I) groups. C) Protein extracts from cell lysates of sorted CD11b⁺Gr-1⁺ splenocytes were analyzed by WES for iNOS, Arg-1 and IDO. α-tubulin was used as a control. Data are representative images or expressed as the mean ± SD of each group. Quantitative analysis was performed using Compass software (Protein Simple). **P < 0.01; ***P < 0.001