Figure 1 –

Black dots overview, manufacturing, and characterization. (A) Principle of black dots, where tension from an adhered cell causes the pattern of dots to displace. (B) Manufacturing black dots substrates using microcontact printing and a sacrificial PVA film to transfer an array of fiducial markers from a patterned stamp to a soft substrate. (C) Example of final manufactured substrate that can be made in the desired fluorescent channel using different fluorescent BSA such as BSA-Alexa Fluor 488 (green), BSA-Alexa Fluor 594 (orange), and BSA-Alexa Fluor 647 (red). The black dotted line area is shown on the right, scaled up 4X larger. (D) Characterization of diameter, center-center spacing, and circularity of black dots. μ = mean, σ = standard deviation. Data from 25,081 individual dots from 2 substrates. Y-axis is Probability Density for all three plots. Normal Gaussian probability density functions are overlayed.