Table 1. Multivariale mixed effects model summary shows significant interaction effects.
The estimate column indicates the expected increase in force (in nN) if the variable in that row increases by 1 μm2 (area) and/or 0.01 units of circularity or F-actin dispersion while all other factors remain constant. The standard error column indicates the error of that estimate. From these estimates and standard errors, p-values are calculated to determine what factors and interactions have a significant (p < 0.05) effect on force. In addition to these individual effects, pairs of effects were tested for interactions, where a positive value in the estimate column indicates a cooperative effect and a negative value an antagonistic effect.
| Estimate | Std. Error | p-value | |
|---|---|---|---|
| Area [nN/μm2] | 0.4100 | 0.0209 | < 0.001 |
| Circularity [nN/0.01 units] | 0.0687 | 0.0247 | 0.0053 |
| F-actin dispersion [nN/0.01 units] | 0.1488 | 0.0187 | < 0.001 |
| Circularity: F-actin dispersion [nN/(0.01 units*0.01 units)] | 0.0024 | 0.0010 | 0.0134 |
| Area: F-actin dispersion [nN/(μm2*0.01 units)] | 0.0024 | 0.0008 | 0.0028 |
| Area: Circularity [nN/(μm2*0.01 units)] | −0.0012 | 0.0009 | 0.2079 |