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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Biomater Adv. 2021 Nov 13;134:112548. doi: 10.1016/j.msec.2021.112548

Figure 5.

Figure 5.

Titanium with nanotopography (Ti Nano) attenuate osteoclast-induced disruption of osteoblast differentiation by reducing the methylated histone accumulation. Protein expression of H3K9me2 (A), H327me3 (B) and EZH2 (C) of osteoblasts (OB) grown on polished titanium (Ti Control), osteoblasts grown in the presence of osteoclasts (OC→OB) on Ti Control, OB grown on Ti Nano and OC→OB on Ti Nano. Binding of the H3K9me2 and H3K27me3 to the promoter regions of the bone markers Runx2, Alpl, Ibsp, Id3, Bglap and Jund in OB grown on Ti Control, OC→OB on Ti Control, OB grown on Ti Nano and OC→OB on Ti Nano (D-H). Representative values from the PCR quantification of the genes, Runx2, Alpl, Ibsp and Id3, regulated by histone methylation (E-H). NC lanes represent samples immunoprecipitated with anti-IgG antibody, INPUT samples consist of total DNA, and H3K9me2 and H3K27me3 lanes refer to DNA immunoprecipitated with anti-H3K9me2 and anti-H3K27me3 antibodies (D). The data of protein expression (n = 3) are presented as mean ± standard deviation and asterisks (*) indicate statistically significant differences (p ≤ 0.05).