FIGURE 2.

Fluorescence properties of cell-free synthesized reporter proteins. (A) Live measurement of fluorescence in the cell-free translation mix of various proteins during cell-free protein synthesis in the FAM channel (top) and the Texas Red channel (bottom). (B) Correlation of dilution of the cell-free translation mix and NTC subtracted signal in the FAM channel (top) and the Texas Red channel (bottom). (C) Correlation of cell-free translation mix reaction volume and NTC subtracted signal in the FAM channel (top) and the Texas Red channel (bottom). Mean values of triplicates are plotted with error bars representing their standard deviation.