TABLE 1.
Code | Amino acid replaced by AzF | Location |
---|---|---|
amb5 | Q5 | VH (FR1) |
amb15 | S15 | VH (FR1) |
amb54 | Y54 | VH (CDR2) |
amb58 | N58 | VH (CDR2) |
amb102 | V102 | VH (CDR3) |
amb138 | G138 | Linker VH-VL |
amb251 | G251 | Linker VL-TS |
amb5+15 | Q5, S15 | VH (FR1) |
amb58+102 | N58, V102 | VH (CDR2), VH (CDR3) |
amb5+15+138 | Q5, S15, G138 | VH (FR1), Linker VH-VL |
Abbreviations: VH, heavy chain variable domain; VL, light chain variable domain; FR, framework region; CDR, complementary determining region; TS, Twin-Strep tag.