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. 2022 Apr 29;10:873906. doi: 10.3389/fbioe.2022.873906

TABLE 1.

Nomenclature of panitumumab scFv amber mutants and ncAA location.

Code Amino acid replaced by AzF Location
amb5 Q5 VH (FR1)
amb15 S15 VH (FR1)
amb54 Y54 VH (CDR2)
amb58 N58 VH (CDR2)
amb102 V102 VH (CDR3)
amb138 G138 Linker VH-VL
amb251 G251 Linker VL-TS
amb5+15 Q5, S15 VH (FR1)
amb58+102 N58, V102 VH (CDR2), VH (CDR3)
amb5+15+138 Q5, S15, G138 VH (FR1), Linker VH-VL

Abbreviations: VH, heavy chain variable domain; VL, light chain variable domain; FR, framework region; CDR, complementary determining region; TS, Twin-Strep tag.