Table 1.

Novel non-synonymous SNPs at the sentinel sites. All novel mutations have been cited under the domains of the kelch propeller region showing the codons with the mutation, the base changes and the sentinel site where they were observed.

Domains	Codons	Reference amino acid	Observed amino acid	Mutation	Specific base change	Site
BTB/POZ	404	R	G	AGA → GGA	A → G	Wa
	411	R	K	AGA → AAA	G → A	Hohoe
	412	N	I	AAT → ATC	AT → TC	Navrongo
	412	N	S	AAT → AGT	A → G	Begoro
	413	P	H	CCG → CAC	CG → AC	Hohoe
	422	L	F	TTC → CTC	T → C	Wa
	431	E	Q	GAA → CAA	G → C	Hohoe
	432	A	T	GCA → ACA	G → A	Hohoe
	442	F	L	TTC → CTC	T → C	Hohoe
Blade 1	445	V	L	GTA → CTA	G → C	Begoro, Wa
	455	E	K	GAA → AAA	G → A	Hohoe, Koforidua, Wa
	456	Y	N	TAT → AAT	T → A	Accra
	458	N	D	AAT → GAT	A → G	Koforidua
	458	N	I	AAT → ATT	A → T	Koforidua
	458	N	H	AAT → CAT	A → C	Koforidua
	461	E	G	GAA → GGA	A → G	Accra
	469	C	R	TGC → CGC	T → C	Cape Coast
	470	W	R	TGG → CGG	T → C	Cape Coast
	473	C	W	TGT → TGG	T → G	Cape Coast
	473	C	S	TGT → TCT	G → C	Hohoe
Blade 2	485	S	G	AGT → GGT	A → G	Koforidua
	488	L	F	TTG → TTC	G → C	Cape Coast, Hohoe
	494	V	I	GTT → ATT	G → A	Hohoe
	499	N	T	AAC → ACC	A → C	Hohoe
	510	V	G	GTG → GGG	T → G	Navrongo
	510	V	L	GTG → TTG	G → T	Wa
	522	S	R	AGT → AGG	T → G	Cape Coast
Blade 3	523	N	K	AAT → AAA	T → A	Sunyani
	529	R	K	AGA → AAA	G → A	Cape Coast
	529	R	I	AGA → ATA	G → T	Koforidua
	530	N	I	AAT → ATT	A → T	Sunyani
	530	N	S	AAT → TCA	AAT → TCA	Koforidua
	532	C	S	TGT → AGT	T → A	Begoro
	536	S	A	TCA → GCA	T → G	Bekwai
	547	D	E	GAT → GAA	T → A	Cape Coast
	547	D	N	GAT → AAT	G → A	Hohoe
	554	N	P	AAT → CCT	AA → CC	Accra
	554	N	T	AAT → ACT	A → C	Hohoe
	555	V	A	GTA → GCA	T → C	Sunyani
	556	E	D	GAA → GAT	A → T	Sunyani
	559	D	Y	GAT → TAT	G → T	Navrongo
	565	W	R	TGG → CGC	TG → CC	Begoro
	569	A	P	GCA → CCA	G → C	Wa
Blade 4	578	A	P	GCT → CCT	G → C	Wa
	579	M	T	ATG → ACG	T → C	Koforidua
	579	M	Y	ATG → TAT	ATG → TAT	Cape Coast
	580	C	V	TGT → GTG	TGT → GTG	Cape Coast
	580	C	R	TGT → CGT	T → C	Begoro
	584	D	L	GAT → TTG	GAT → TTG	Koforidua
	585	N	H	AAT → CAT	A → C	Hohoe
	585	N	I	AAT → ATA	AT → TA	Koforidua
	587	I	T	ATT → ACT	T → C	Cape Coast
	587	I	N	ATT → AAT	T → A	Cape Coast, Koforidua
	590	I	T	ATT → ACT	T → C	Navrongo
	598	L	I	TTA → ATA	T → A	Bekwai
	605	E	D	GAA → GAC	A → C	Cape Coast
	615	P	L	CCA → CTA	C → T	Cape Coast
Blade 5	616	Y	H	TAT → CAT	T → C	Koforidua
	619	L	V	TTA → GTA	T → G	Begoro
	623	S	N	AGT → AAT	G → A	Cape Coast
	628	F	L	TTT → CTT	T → C	Cape Coast
	628	F	L	TTT → TTA	T → A	Hohoe, Koforidua
	633	Q	H	CAA → CAT	A → T	Accra
	640	I	F	ATT → TTT	A → T	Cape Coast
	640	I	S	ATT → AGT	T → G	Begoro
	643	E	D	GAA → GAC	A → C	Cape Coast
	646	I	K	ATA → AAA	T → A	Cape Coast
	648	D	Y	GAT → TAT	G → T	Sunyani
	661	Q	L	CAA → CTA	A → T	Cape Coast, Koforidua
	661	Q	G	CAA → GCA	CA → GC	Koforidua
	664	N	H	AAT → CAT	A → C	Cape Coast
Blade 6	668	E	D	GAG → GAT	G → T	Cape Coast, Koforidua
	669	K	E	AAA → GAA	A → G	Cape Coast, Koforidua
	669	K	N	AAA → AAC	A → C	Koforidua
	672	N	I	AAT → ATT	A → T	Cape Coast, Koforidua
	690	G	D	GGC → GAC	G → A	Cape Coast, Koforidua
	692	V	L	GTT → CTT	G → C	Cape Coast
	696	C	S	TGT → AGT	T → A	Cape Coast