Table 2.

Summary of computational analysis of DNA polymorphisms found in pfk13 in Ghanaian isolates by location, year and ecological zones. The computational analysis of the sequences to reveal the nucleotide diversity of the mutations in the pfk13 gene in Ghanaian isolates for study sites, year and ecological zones.

	π	S	Tajima's D	T’s D p value	No. of haplotypes/haplotype diversity (h/Hd)
A. Location
Accra	0.00395	136	− 2.70944	< 0.001	29 (0.926)
Begoro	0.00418	222	− 2.80559	< 0.001	30 (0.759)
Bekwai	0.00589	232	− 2.66217	< 0.001	28 (0.754)
CapeCoast	0.00315	247	− 2.87368	< 0.001	48 (0.722)
Hohoe	0.00154	138	− 2.89841	< 0.001	44 (0.686)
Koforidua	0.00096	102	− 2.70784	< 0.001	28 (0.472)
Navrongo	0.00299	276	− 2.91636	< 0.001	41 (0.607)
Sunyani	0.00415	164	− 2.64914	< 0.001	22 (0.614)
Tarkwa	0.01467	89	− 1.15583	> 0.10	6 (1.000)
Wa	0.00303	87	− 2.55226	< 0.001	24 (0.906)
Total	0.00383	1034	− 2.85874	< 0.001	307 (0.6887)
B. Year
2007	0.00919	305	− 2.78904	< 0.001	23 (0.829)
2010	0.00798	344	− 2.74562	< 0.001	32 (0.666)
2012	0.00255	132	− 2.80328	< 0.001	18 (0.468)
2014	0.00688	255	− 2.57399	< 0.001	51 (0.879)
2016	0.00289	767	− 2.9292	< 0.001	186 (0.7127)
2017	0.00097	102	− 2.92075	< 0.001	28 (0.474)
Total	0.00383	1034	− 2.85874	< 0.001	307 (0.6887)
C. Ecological zones
Coastal	0.00504	460	− 2.9099	< 0.001	82 (0.827)
Forest	0.00326	706	− 2.88742	< 0.001	159 (0.6300)
Savannah	0.00458	491	− 2.94234	< 0.001	71 (0.731)
Total	0.00383	1034	− 2.85874	< 0.001	307 (0.6887)

S—number of segregating sites in the gene; π—nucleotide diversity at the gene locus.