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. 2022 May 12;13:2652. doi: 10.1038/s41467-022-30249-z

Fig. 4. Demonstration of the bifunctional reaction system for the identification of C=C bonds location and E-Z configuration in polyunsaturated lipids.

Fig. 4

a Mass spectrum of the photocatalytic reaction solution of FA 18:2 (9Z, 12Z). b MS/MS spectrum of the sodiated photocycloaddition product (P1/P2) of FA 18:2 (9Z, 12Z). c Mass spectrum of the photocatalytic reaction solution of PC 18:1 (9Z)/18:1 (9Z). d MS/MS spectrum of the protonated photocycloaddition product (P1/P2) of PC 18:1 (9Z)/18:1 (9Z). Assignment of the fragments: m/z 808.51, [P1a + H]+; m/z 785.59, [PC]+; m/z 686.40, [P1/P2 − FA 18:1]+; m/z 676.45, [P2a + H]+; m/z 668.40, [P1/P2 − FA 18:1 − H2O]+; m/z 625.44, [P1a − C5H14NO4P (183)]+; m/z 522.36, [PC − FA 18:1]+; m/z 504.34, [PC − FA 18:1 − H2O]+; m/z 451.34, [P2a − C5H14NO4P (183)]+. e EICs of FA 18:2 (9Z, 12Z) and FA 18:2 (9Z, 11E) before and after the photocatalytic reaction at m/z 279.2 in negative ion mode. f EICs of PC 18:1 (9Z)/18:1 (9Z) and PC 18:0/18:2 (9Z, 12Z) before and after the photocatalytic reaction at m/z 786.6 in positive ion mode.