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. 2022 May 12;12:7853. doi: 10.1038/s41598-022-11566-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The in vitro development of follicles at various stages (Scale bar, 100 mµ). On D2, the arrow head indicated the outgrowth of the adhered follicular cells; On D10, the arrow head indicated the follicular antrum; In the picture of ovulation, the arrows indicated the ovulated cumulus-oocyte complexes (CoCs); and in the picture of M II oocytes, the arrows indicated the first polar body excreted by mature oocytes.