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. 2022 May 12;12:7853. doi: 10.1038/s41598-022-11566-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of mitochondrial membrane potential in oocytes. (A) JC-1 probe was used to detect the mitochondrial membrane potential in oocytes. JC-1 forms J-aggregates and produces red fluorescence under normal mitochondrial membrane potential (Scale bar, 200 μm). Under reduced or lost mitochondrial membrane potential, JC-1 exists in J-monomers and produces green fluorescence. (B) The average fluorescence intensity was analyzed in oocytes (N = 10). Compared with control, ^aP < 0.05; and compared with BPA group, ^bP < 0.05.