Table 2.
Primer sequences for quantitative real-time PCR.
| Gene | Primer sequences (5′to 3′) | Annealing temperature | |
|---|---|---|---|
| Forward | Reverse | ||
| CAT | TTACCCCAACAGCTTCAGCGCA | GGCAATGTTCTCACACAGGCGT | 58 |
| SOD1 | AGCATGGGTTCCACGTCCATCA | ACCGTCCTTTCCAGCAGTCACA | 58 |
| SOD2 | ATAATGTTGTGTCGGGCGGCGT | TCGGTGGCGTTGAGATTGTTCACG | 60 |
| Caspase 3 | TGGCATTGAGACAGACAGTGGGA | TGCGCGTACAGCTTCAGCAT | 57 |
| Cathepsin B | TGGCAAGATTTGGACGACTGGACC | ACAGCAGGCACTACAAACCGCA | 60 |
| Bcl-2 | ACCGTCGTGACTTCGCAGAGAT | TGTGCAGATGCCGGTTCAGGTA | 58 |
| β-actin | TGTTACCAACTGGGACGACA | CTGGGTCA TCTTTTCACGGT | 58 |
CAT, catalase; SOD1, superoxide dismutase 1; SOD2, superoxide dismutase 2; and Bcl-2, B-cell lymphoma-2.