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. 2022 May 12;13:2635. doi: 10.1038/s41467-022-30301-y

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative single-molecule kymographs of Cy5-FtsA WT (green) and Cy5-FtsA R286W (cyan) at increasing concentrations. b With higher concentrations the mobility of both proteins decreases and the residence time increases, but R286W remains 10x more mobile and has a 2-7x lower lifetime. Thick line indicates the mean value, shaded area represents 95% confidence interval; n = 3. c Schematic of the experiment to measure FRET between Cy3-FtsA (donor) and Cy5-FtsA (acceptor). d, e Representative montage of acceptor photobleaching experiment with FtsA WT (d) or R286W (e). Scale bars are 5 µm. f Kymographs from acceptor photobleaching experiments, and corresponding donor signal for FtsA WT (green) and R286W (cyan). Scale bars are 4 µm and 20 s, respectively. g Representative curves showing acceptor (top) and donor fluorescence intensities (bottom) for FtsA WT (green) and R286W (cyan). h Left: FtsA WT FRET increases at higher concentrations, FtsZ slightly decreases, while FtsN_cyto and FtsZ/FtsN_cyto increase FtsA self-interaction. Right: FtsA R286W FRET increases with concentration, but is significantly lower than for WT. FtsZ and FtsN_cyto increase FRET, and with both the FRET signal of WT and R286W are indistinguishable. i Left: Off-rates remain slow with increasing concentrations of WT. FtsN_cyto and FtsN_cyto/FtsZ further decrease the exchange. Right: Off-binding rates remain constantly high with increasing concentrations of FtsA R286W. FtsN_cyto and FtsN_cyto/FtsZ decrease the rate, but it remains higher than for WT. j Left: D_coeff drops with increasing FtsA WT concentrations and decreases further with FtsZ and FtsN_cyto. Right: D_coeff remains unchanged at increased concentrations of FtsA R286W. FtsN_cyto and FtsZ slow down R286W, which remains more mobile than WT. Dots in all plots represent independent experiments, thick lines indicate the mean and error bars depict the standard deviation. Source data are provided as a Source Data file.