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. 2022 Apr 29;13:890520. doi: 10.3389/fphys.2022.890520

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Copyright © 2022 Liu, Pan, Zhao, Guo, Qiu, Balasubramanian and Jha.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of heat stress on cecal metabolome in indigenous broilers (n = 5/group). (A) Orthogonal partial least squares discriminant analysis (OPLS-DA) under negative ions mode (NEG); (B) OPLS-DA under positive ions mode (POS); (C) Cluster heatmap analysis of samples under positive ions mode (POS); (D) Cluster heatmap analysis of samples under negative ions mode (NEG); (E) Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways enrichment analysis; NT, normal treatment group (21.3 ± 1.2°C, 24 h/day), HS, heat stress group (32.5 ± 1.4°C, 8 h/day).