TABLE 4.
Amino acid production under repression and induction conditions by SG15 and SG183 transformed with pCHA-R2
| ta (h) and strain | Intracellular (extracellular) threonine concn (nmol/mg [dry wt]) with the following concn of serine (g/liter) added to the medium:
|
||||
|---|---|---|---|---|---|
| 0 | 1 | 0.01b | 0.1b | 1b | |
| 14 | |||||
| SG15 | 37 (NEc) | 100 (80) | |||
| SG183 | 49 (NDd) | 35 (30) | |||
| 15 | |||||
| SG15 | 41 (NE) | 99 (78) | 40 (NE) | 37 (NE) | 40 (NE) |
| SG183 | 48 (3) | 38 (31) | 48 (4) | 47 (4) | 47 (4) |
| 17 | |||||
| SG15 | 40 (NE) | 118 (79) | 34 (NE) | 35 (NE) | 33 (NE) |
| SG183 | 60 (10) | 49 (34) | 61 (7) | 65 (11) | 68 (8) |
| 38 | |||||
| SG15 | 58 (NE) | 112 (77) | 78 (NE) | 57 (NE) | 70 (NE) |
| SG183 | 99 (27) | 67 (40) | 161 (44) | 194 (55) | 226 (67) |
a
Cultivation time.
b
Serine was added to 14-h cultures.
c
NE, not estimated.
d
ND, not detectable.