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. 2022 Apr 29;16:863793. doi: 10.3389/fnins.2022.863793

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Copyright © 2022 Talifu, Qin, Xin, Chen, Liu, Dangol, Ma, Gong, Pei, Yu, Li and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental protocol and time points. After adaptive training for 1 week in advance, the rats were randomly divided into four groups for spinal cord injury modeling or sham operation. Two groups were injected with adeno-associated viral (AAV)-N-T3/AAV-IGF-1 or AAV-GFP/AAV-RFP 1 day after surgery. Three rats were then examined by immunofluorescence 4 weeks after surgery to determine the expression of AAV. The other rats received behavioral and electrophysiological evaluations for 10 weeks. After 70 days, all rats were sacrificed for western blotting and immunofluorescence detection.