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. 2022 Apr 29;16:863793. doi: 10.3389/fnins.2022.863793

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Copyright © 2022 Talifu, Qin, Xin, Chen, Liu, Dangol, Ma, Gong, Pei, Yu, Li and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of KCC2 in spinal motoneurons. (A) Immunofluorescence staining of KCC2 and ChAT in the spinal cord of rats from different groups. (B) Co-localization immunofluorescence intensity of KCC2 and motoneurons, six rats per group (309 ChAT-positive neurons in 22 visual fields in the sham group, 372 ChAT-positive neurons in 23 visual fields in the SCI group, 404 ChAT-positive neurons in 23 visual fields in the SCI-N + I group). *Represents the significance of the difference between groups (one-way ANOVA, Bonferroni’s post hoc test), One, two, and three symbols indicate P < 0.05, P < 0.01, and P < 0.001, respectively.